Research Paper Volume 15, Issue 11 pp 4667—4684

Identification of novel genes associated with exercise and calorie restriction effects in skeletal muscle


Figure 3. Selected genes do not regulate starvation-induced autophagy. (A) C2C12 myotube cells were transfected with selected genes or control siRNAs and exposed to EBSS for 6 h to induce autophagy. The autophagy markers LC3B-1/2 and P62 were measured in protein extracts using western blot analysis; GAPDH and HSP90 were used as loading controls. (B) The insulin signaling molecules p-AKT and AKT (C) were quantified using western blot analysis and densitometry (n = 3). (D) Glucose uptake in C2C12 myotubes transfected with selected genes (n = 3). All data are represented as mean ± SEM. Statistically significant differences are denoted as *p < 0.05, **p < 0.01, ***p < 0.005.