Research Paper Volume 15, Issue 11 pp 4667—4684

Identification of novel genes associated with exercise and calorie restriction effects in skeletal muscle


Figure 4. Selected genes regulate mitochondrial respiration. (A) The real-time oxygen consumption rate (OCR) of selected gene-transfected C2C12 myotubes was determined using a Seahorse XFe96 analyzer. (B) The area under the curve (AUC) values of basal respiration, ATP-linked respiration, and maximal mitochondrial respiration. OCRs were normalized to total cellular protein. All data are represented as mean ± SEM. Statistically significant differences are denoted as *p < 0.05, **p < 0.01, ***p < 0.005.