Research Paper Volume 15, Issue 15 pp 7794—7810
Hmgcs2 regulates M2 polarization of macrophages to repair myocardial injury induced by sepsis
Figure 6. Hmgcs2 affects cardiomyocyte activity via macrophage polarization. (A) CCK-8 experiments showed the effects of different groups of macrophages on the activity of cardiomyocytes after co-culture with cardiomyocytes. (B) Flow cytometry experiments showed the effects of different groups of macrophages on cardiomyocyte apoptosis after co-culture with cardiomyocytes. Abbreviations: NC: control group; LPS: LPS induced M1 type macrophages; LPS+ empty: M1 macrophage group transfected with empty plasmid; LPS+Hmgcs2: Hmgcs2 over-expressing M1 macrophage group; LPS+Hmgcs2+PP1: M1 macrophages with over-expression of Hmgcs2 based on PP1 administration; LPS+IL-4: LPS+IL-4 induced M2 type macrophages. *P < 0.05, **P < 0.01, ***P < 0.001: vs. NC group; #P < 0.05, ##P < 0.01, ###P < 0.001: vs. LPS + HMGCS2 group.