Research Paper Volume 15, Issue 18 pp 9572—9589

Core fucosylation regulates alveolar epithelial cells senescence through activating of transforming growth factor-β pathway in pulmonary fibrosis


Figure 5. Inhibition of TGF-βR I activity with EW-7197 abrogates overexpression of CF modification induced AECs senescence. MLE12 cells were infected with an adenovirus expression vector overexpressing FUT8 and then stimulated with EW-7197 for 6 hours. (A) To test the effect of gene overexpression, we used RT-PCR to show FUT8 mRNA expression in MLE12 cells infected with FUT8-OE or NT-OE. ***P < 0.001 compared with NT OE group (Unpaired t-test). (BF) Western blotting using anti-p-Smad2/3, anti- FUT8, anti-p16, anti-p21 and anti-β-actin antibodies. Densitometric analyses of the bands were adjusted to be equal to β-actin. Data were presented as the mean ± SD. *P < 0.05. **P < 0.01. ***P < 0.001. ****P < 0.0001. (G, H) SA-β-gal staining shows indicative pictures. Scale bar, 100 μm. Data were presented as the mean ± SD. **P < 0.01. ****P < 0.0001.