Research Paper Volume 15, Issue 18 pp 9590—9613

YY1 is regulated by ALKBH5-mediated m6A modification and promotes autophagy and cancer progression through targeting ATG4B


Figure 3. YY1 facilitates ATG4B expression. (A) The analysis of the overlap (Venn diagram) between YY1 target genes and YY1-related genes revealing the four targets involved in the YY1 regulation of autophagy-related genes. (B) Heatmap showing the expression features of those four genes in GC and adjacent normal tissues from the Xena platform. (C) A correlation test validating the association between ATG4B and YY1 on the Xena platform. (D) qRT-PCR assay displaying the expression levels of ATG4B in GC and GES-1 cells. (E) ChIP assay unveiling YY1 enrichment on the ATG4B promoter in MGC803 and AGS cells stably transfected with Ca-NC, Ca-YY1, Ci-NC or Ci-YY1. (F) Schematic illustration showing the YY1 binding sites on the ATG4B promoter at -135/-121 bases from UCSC Genome Browser, and the wild-type (WT) or mutations (Mut) of ATG4B promoter reporters were designed. (G) Dual-luciferase assay disclosing the luciferase activity of ATG4B promoter in AGS and MGC803 cells stably transfected with Ca-NC, Ca-YY1, Ci-NC or Ci-YY1. (H, I) qRT-PCR and western blot assays showing the transcript and protein expression levels of ATG4B in MGC803 and AGS with YY1 overexpression or knockdown. *P < 0.05 vs. GES-1, Ca-NC, Ci-NC.