Research Paper Volume 15, Issue 18 pp 9590—9613

YY1 is regulated by ALKBH5-mediated m6A modification and promotes autophagy and cancer progression through targeting ATG4B


Figure 5. The m6A-dependent promotion of YY1 is positively associated with YTHDF1. (AD) MeRIP, qRT-PCR, dual luciferase and western blot assays displaying the m6A and mRNA levels, as well as the 3'-UTR activity and protein expression of YY1 in MGC803 and AGS cells transfected with Ca-YTHDF1 or Ci-YTHDF1 and co-transfected with Ca-ALKBH5 or Ci-ALKBH5. (E, F) qRT-PCR assay showing the half-life levels of YY1 mRNA in AGS cells treated with actinomycin D (1 μg/ml) at the indicated periods. *P < 0.05 vs. Ca-NC, Ci-NC.