Research Paper Volume 15, Issue 18 pp 9676—9694

Ferroptosis and WDFY4 as novel targets for immunotherapy of lung adenocarcinoma


Figure 8. The effect of WDFY4 on the biological characteristics of lung adenocarcinoma A549 cells in vitro and in vivo. (A) The expression level of WDFY4 in A549 lung adenocarcinoma cells transfected with ad-Ctrl and ad-WDFY4. (B) Cell viability was measured by CCK-8 assays. (C) Cells were collected and stained with PI and annexin V-FITC for apoptosis analysis by flow cytometry. (DF) Scratch wound healing and Transwell migration assay analyzed the metastasis of A549 cells after transfection of ad-WDFY4. (G) A549 cells transfected with ad-Ctrl and ad-WDFY4 were transplanted subcutaneously into nude mice, and the cancer growth curve and cancer weight were monitored (n = 5). (H, I) The expression levels of Ki-67 and Caspase-3 in cancer tissues were analyzed by immunohistochemistry (n = 5).