Research Paper Volume 15, Issue 18 pp 9743—9758

NOTCH2 gene mutation and gamma-secretase inhibitor in mediating the malignancy of ovarian cancer


Figure 3. Impacts of NOTCH2 P2113S on A2780 and SKOV3 cell proliferation, migration, and invasion. (A) Notch2 level was tested following transfection with NOTCH2-P2113S in A2780 and SKOV3 cells; (B) CCK-8 assay was used for detecting cell proliferation (*p<0.05 vs empty vector); (C) Scratch wound-healing assay showed alterations of cell migration. Upper panel, representative images (×100); Lower panel, quantitative analysis (*p<0.05); (D) Transwell chamber assay was used for evaluating cell invasion by NOTCH2 P2113S transfection. Upper panel, representative images (×100); lower panel, quantitative analysis (*p<0.05). (E) EdU labeling test was used for testing cell proliferation. N=3.