Research Paper Volume 15, Issue 19 pp 10105—10116

SNHG25 promotes colorectal cancer metastasis by regulating MMP2


Figure 4. The expression of MMP2 is regulated by SNHG25 in CRC. (A) Volcano plot generate from the RNA-seq data showed the statistical significance (P value) and magnitude of change (fold change) of gene expression after SNHG25 knockdown. (B, C) The expression of MMP2 was detected by qRT-PCR or western blot in HCT-116 and HT-29 cells after SNHG25 knockdown. (D) GSEA results demonstrate the correlation between SNHG25 and gene sets related to metastasis (ALONSO_METASTASIS_EMT_UP, BIDUS_METASTASIS_UP and CHANDRAN_METASTASIS_TOP50_UP). (E) Representative FISH images demonstrated subcellar location of SNHG25 in HCT-116 and HT-29 cells (red). Nuclei were stained by DAPI (blue). (F) Relative SNHG25 expression in the nuclear and cytoplasm fractions of HCT-116 and HT-29 cells. Nuclear controls: U6, cytosolic controls: GAPDH. **P < 0.01 and ***P < 0.001.