Research Paper Volume 15, Issue 19 pp 10291—10306

Dendrobium officinale polysaccharide decreases podocyte injury in diabetic nephropathy by regulating IRS-1/AKT signal and promoting mitophagy


Figure 2. DOP treatment distinctly relived HG-induced oxidative stress and IR of MPC5 cells. (A) To determine the safety dosage of DOP, MPC5 cells were treated with various dosages of DOP (0, 5, 10, 25, 50, 100 and 250 μg/ml) for 48 hours. Cell viability was assessed by CCK-8 method. (B) DOP treatment notably augmented HG-reduced glucose consumption of MPC5 cells. (C) DOP treatment remarkably reduced HG-increased generations of malondialdehyde (MDA). (D, E) DOP treatment markedly relived HG-induced suppressive effects on the phosphorylation of insulin receptor substrate (IRS)-1 (phospho Y632), AKT (phospho Ser473), AKT (phospho Thr308) and Nephrin. (F) HG inducement markedly augmented the formation of γ-H2A.X foci, while DOP treatment notably reversed this effect. The bar in the figure indicates 10 μm (*p < 0.05; ** p < 0.01; *** p < 0.001, n=3).