Research Paper Volume 15, Issue 19 pp 10291—10306

Dendrobium officinale polysaccharide decreases podocyte injury in diabetic nephropathy by regulating IRS-1/AKT signal and promoting mitophagy


Figure 6. DOP alleviated the injury of podocytes in the diabetic mice. In this study, C57BL/6 mice were randomly allocated to three groups: control, STZ and STZ+DOP groups with six mice in each group. In STZ and STZ+DOP groups, C57BL/6J mice were injected intraperitoneally with STZ for 5 consecutive days. Two weeks after diabetes was induced, the mice in STZ+DOP group were injected with DOP (10mg/kg) intraperitoneally once a day for eight consecutive weeks. After the ten weeks, the blood was retained and the urine during 24h was taken from all the mice. (A) The urine albumin and blood creatinine were assessed for the evaluation of the albumin/creatinine ratio. (B) Blood urea nitrogen (BUN) was determined using the Urea Nitrogen (BUN) Colorimetric Detection Kit. (C) Immunofluorescence analysis of renal tissue was conducted to assess Nephrin protein level (*** p < 0.001; n=6).