Research Paper Volume 15, Issue 19 pp 10524—10539
Paroxetine protects against bleomycin-induced pulmonary fibrosis by blocking GRK2/Smad3 pathway
Figure 7. Smad3 overexpression offset PRXT-elicited protection against TGF-β1-induced activation of lung fibroblasts. (A) Representative western blotting images of Smad3 in primary lung fibroblasts transfected with Ad-Smad3. (B) Cell viability in the indicated groups (n=5). (C, D) Relative mRNA expression levels of Collagen I and α-SMA in the indicated groups (n=5). (E, F) SOD activity and NADPH oxidase activity (n=5). (G, H) Representative western blotting images of GRK2 and its quantification in the indicated groups (n=5). (I) Chemical structure of PRXT and the possible mechanisms of the protective effects of PRXT in pulmonary fibrosis. Arrowheads denote the upregulate while the “T” ending lines represent downregulate.