Research Paper Volume 16, Issue 2 pp 1620—1639

PROX1 interaction with α-SMA-rich cancer-associated fibroblasts facilitates colorectal cancer progression and correlates with poor clinical outcomes and therapeutic resistance


Figure 2. Effect of PROX1 knockdown on CRC malignant phenotype. (A) The mRNA expression level of PROX1 after shRNA transfection was evaluated by RT-PCR. (B) CCK-8 assay of sh-NC and PROX1 shRNA #1- transfected in HCT116 and SW 620 cells. (C) Colony formation assay analysis for the inhibitory effect of PROX1 knockdown on HCT116 and SW 620 cell migration. (D) Flow cytometry analysis of cell cycle phases distribution of HCT116 and SW 620 cells following the PROX1-shRNA infection. The experiments were performed thrice, and the data are presented as the mean ± standard deviation. *P < 0.05 and **P < 0.01 vs. sh-NC.