Research Paper Volume 16, Issue 2 pp 1620—1639

PROX1 interaction with α-SMA-rich cancer-associated fibroblasts facilitates colorectal cancer progression and correlates with poor clinical outcomes and therapeutic resistance


Figure 3. PROX1 knockdown reduces the migration and invasiveness of SW620 and HCT116 cells. (A) Wound healing assay for the inhibitory effect of PROX1 knockdown on HCT116 and SW620 cell migration. Cell migration into the wounded area was quantified based on the dashed line as time 0. Images were taken immediately after scratching, 0 h and 16 h later. Original magnification, ×200. Transwell invasion assay shows the invasive ability of (B) HCT116 and SW-620, significantly suppressed in PROX1 shRNA-infected cells. (C) RT-PCR shows PROX1 knockdown suppressed transcript expression of Fibronectin and Snail while upregulating E-cadherin and Connexin 26. GAPDH is used as an internal control. Data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***P < 0.001.