Research Paper Volume 16, Issue 2 pp 1620—1639

PROX1 interaction with α-SMA-rich cancer-associated fibroblasts facilitates colorectal cancer progression and correlates with poor clinical outcomes and therapeutic resistance


Figure 4. PROX1 overexpression promotes the growth, migration, and invasion of colon adenocarcinoma cells. HT-29 cells were transfected with either the pcDNA-PROX1 overexpressing vector or empty vector control. (A) RT-qPCR and (B) proliferation and (C) colony formation capability were estimated by CCK-8 and colony formation assays, respectively. (D) Flow cytometry showed the distribution of pcDNA-PROX1-transfected HT-29 cells in the G1, S, and G2/M phases of the cell cycle. (E) Cell migration ability was assessed by wound healing and (F) Invasion was assessed by the Transwell invasion assay in pcDNA-PROX1-transfected HT-29 cells and control cells. Magnification, ×100. Scale bar, 100 μm. The experiments were performed three times, and the data are presented as the mean ± standard deviation. *P < 0.05 and **P < 0.01 vs. NC.