Figure 7. Silencing of SIRT1 abolished the inhibitory effects of PHN-20 on NLRP3 signaling in hypoxia-treated PMECs. PMECs were transduced with lenti-viral SIRT1 shRNA, followed by stimulation with hypoxia in the presence or absence of PHN-20 (20 nM) for 24 hours. (A) Western blot revealed successful knockdown of SIRT1; (B) Western blots of NLRP3, ASC. Protein levels of NLRP3 and ASC. (**, P<0.01 vs. control group; #, P<0.05 vs. hypoxia group; ^, P<0.05 vs. PHN-20 group).