Antagomir of miR-31-5p modulates macrophage polarization via the AMPK/SIRT1/NLRP3 signaling pathway to protect against DSS-induced colitis in mice

Yuyi Yuan; Shuangjiao Deng; Jia Yang; Zhexing Shou; Chunzhu Wei; Lijuan Zhang; Feng Zhu; Fei Gao; Xingxing Liu; Yujin Liu; Qianyun Chen; Heng Fan

doi:doi:10.18632/aging.205651

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Research Paper Volume 16, Issue 6 pp 5336—5353

Antagomir of miR-31-5p modulates macrophage polarization via the AMPK/SIRT1/NLRP3 signaling pathway to protect against DSS-induced colitis in mice

Figure 4. mir-31-5p antagomir restored intestinal mucosal tight junction protein and reduced colonic inflammation in DSS-induced colitis mice. (A) Immunofluorescence staining of ZO-1 and occludin in colon tissue (x200 magnification). (B) Quantitative fluorescence analysis of ZO-1 and occludin (ordinate represented the average fluorescence intensity analyzed by ImageJ software). (C) ELISA analysis of the expression of IL-1β, TNF-α, TGF-β and IL-10 in colon. Each bar represents mean ± SD, n ≥ 3 from each group. ns represented P > 0.05, ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001 vs. DSS group.