Antagomir of miR-31-5p modulates macrophage polarization via the AMPK/SIRT1/NLRP3 signaling pathway to protect against DSS-induced colitis in mice

Yuyi Yuan; Shuangjiao Deng; Jia Yang; Zhexing Shou; Chunzhu Wei; Lijuan Zhang; Feng Zhu; Fei Gao; Xingxing Liu; Yujin Liu; Qianyun Chen; Heng Fan

doi:doi:10.18632/aging.205651

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Research Paper Volume 16, Issue 6 pp 5336—5353

Antagomir of miR-31-5p modulates macrophage polarization via the AMPK/SIRT1/NLRP3 signaling pathway to protect against DSS-induced colitis in mice

Figure 7. mir-31-5p antagomir may alleviate colitis in mice by transforming M1-type macrophages into M2-type macrophages. (A) The ratio of M1/M2 in spleen and lymphocytes was determined by flow cytometry. (B) Statistical analysis of M1/M2 ratio in spleen and lymphocytes. (C) The expressions of NOX2, HADHB, iNOS, Arg1 and β-catenin proteins in colon were analyzed by Western blotting. (D) The mRNA expression of M1-type and M2-type macrophage marker genes was detected by qPCR. (E) Statistical analysis of protein expression levels of NOX2/β-actin, HADHB/β-actin, iNOS2/β-actin and Arg1/β-actin. Each bar represents mean ± SD, n ≥ 5 from each group. Ns represented P > 0.05, ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001 vs. DSS group.