Research Paper Volume 16, Issue 8 pp 6809—6838

Comprehensive analysis of macrophage-related genes in prostate cancer by integrated analysis of single-cell and bulk RNA sequencing


Figure 11. Identifying NCF4 as the hub gene. (A) The “mgeneSim” function reveals the hub gene NCF4 in the MRS. (B) The violin diagram shows the expression of NCF4 in various types of cells in GSE141445. Correlation scatter plots demonstrate the correlation of NCF4 expression levels with the content of macrophage cells (C), macrophage M1 cells (D), and macrophage M2 cells (E). Paired box plots demonstrating the difference in NCF4 expression between normal and cancerous prostate tissues in TCGA-PRAD cohort (F) and CPGEA cohort (G). (H) The immunohistochemical staining shows NCF4 expression at the protein level in normal and PCa tissues.