Research Paper Volume 16, Issue 8 pp 6809—6838

Comprehensive analysis of macrophage-related genes in prostate cancer by integrated analysis of single-cell and bulk RNA sequencing


Figure 6. Development of a macrophage-related signature (MRS). (A) Volcano plot of DEGs between macrophage-related molecular subtypes in TCGA cohort. Significant DEGs were found when P < 0.05 and |log2FoldChange|> 1. Genes that are upregulated are shown in red, and genes that are downregulated are shown in blue. (B) Heatmap of DEGs. Bubble plots of the GO (C) and KEGG pathways (D) functional enrichment of DEGs. (E) Based on macrophage-related clusters, LASSO Cox regression analysis selected eight genes. (F) Cross-validation for tuning parameter selection in the LASSO model. (G) Forest plot of multivariate Cox regression result. (H) The Kaplan-Meier curves in the training cohort (TCGA). (I) The AUC at 1-, 3-, and 5-years of prognostic models in the training cohort. (J) ROC curves evaluate the predictive accuracy of the risk score and clinicopathological features. ns, not significant; **P < 0.01; ***P < 0.001.