Research Paper Volume 16, Issue 8 pp 6990—7008

Activating transcription factor 6 alleviates secondary brain injury by increasing cystathionine γ-lyase expression in a rat model of intracerebral hemorrhage


Figure 2. The protein level of ATF6 in neuron increased significantly at 24 h after ICH. (A, B) Western blot analysis and quantification of ATF6 at 1h, 3h, 6h, 12h, 24h and 48h after ICH. (C, D) Double immunofluorescence analysis of ATF6 (green) and neuron (red) in brain sections. Nuclei were labeled with DAPI (blue). Arrow indicated ATF6 positive cells. Scale bar = 50 μm. (E, F) Western blot analysis quantification of CBS and CTH at 1h, 3h, 6h, 12h, 24h and 48h after ICH. The black dots represent individual data in each group. **p < 0.01 and *p < 0.05 vs. sham group, NS, no significant difference vs. Sham group, n = 6.