Research Paper Volume 16, Issue 8 pp 7009—7021

Whole-transcriptome profiling reveals potential biomarkers for the reversal of thymic epithelial cell senescence by umbilical cord mesenchymal stem cells


Figure 5. UCMSCs boost the activity of the PI3K-AKT pathway in senescent TECs. (A) VEGFA expression was detected by ELISA. (B) The PI3K-AKT signaling pathway proteins VEGFA, PI3K, and AKT were shown to be expressed by western blotting, with β-actin serving as a control. (CE) After the PI3K-AKT signaling pathway, immunofluorescence was used to measure the levels of p27, CDK2, and CCNE, which are important for cell cycle regulation (100×). The measured values represent the mean ± standard deviation (X ± s) of three replicate experiments. *P < 0.05, **P < 0.01, ***P < 0.001).