Research Paper Volume 16, Issue 8 pp 6717—6730

Characterization of age-associated gene expression changes in mouse sweat glands


Figure 3. Localization of core secretory proteins in SWGs. (A) Localization of FOXA1 and BEST2 in scattered secretory cells (arrows), ATP6V1B1 in luminal cell membrane in the secretory coil, SERCA3 in the cytoplasm of secretory cells (but not sweat duct, demarcated by broken lines), and VAMP1 protein surrounding KRT8+ secretory cells and co-localizing with the myoepithelial cell marker KRT14. (B) Top, expression of FOXC1 in sweat duct cells (left); higher magnification shows localization of FOXC1 in epidermal and dermal duct luminal cells (middle). FOXC1 protein is expressed in secretory cells (arrows, right), but expression signals are stronger in duct cells (dotted circle). Bottom, negative control staining (secondary antibodies). Scale bars, 25 μm. Data are representative of two to four biological replicates.