Research Paper Volume 16, Issue 9 pp 7870—7888

Leptin attenuates the osteogenic induction potential of BMP9 by increasing β-catenin malonylation modification via Sirt5 down-regulation


Figure 6. The effects of BMP9 on malonylation of β-catenin in C3H10T1/2 cells. (A) Western blot assay shows the effect of BMP9 on lysine malonylation. (B) Semi-quantification of the level of BMP9 or malonylation. (C) IP assay shows the lysine malonylation of β-catenin. (D) LC–MS analysis shows the malonylation proteins in C3H10T1/2. (E) KEGG pathway analysis shows the possible biochemical processes or diseases that may be associated with lysine malonylation. (“*” P < 0.05, “**” P < 0.01).