Research Paper Volume 16, Issue 9 pp 7979—7999

Mechanistic exploration and experimental validation of the Xiaochaihu decoction for the treatment of breast cancer by network pharmacology


Figure 5. Xiaochaihu decoction restrains breast cancer cell proliferation in vitro. (A) The anti-breast cancer activity of Xiaochaihu decoction. The XCH decoction at various concentrations (0, 2.5, 5, 10, 20, 40, 80 or 160 μg/mL) was used to treat the four breast cancer cell lines for 48 hours. Cell viability was determined by a CCK-8 assay. (B, C) The breast cancer cell lines CAL-148 and MCF-7 were treated with different concentrations of the XCH decoction for 24, 48 or 72 hours, and viability was determined using a CCK-8 assay. (D) The cell state was observed under a 40x light microscope. (E) Effect of the XCH decoction on the colony-forming ability of the CAL-148 and MCF-7 cell lines. (F) Quantification of colony number. The data are shown as the mean ± SD of three experiments. *P <0.05, **P < 0.01, ***P < 0.001 compared with the control group.