Research Paper Volume 16, Issue 9 pp 7979—7999
Mechanistic exploration and experimental validation of the Xiaochaihu decoction for the treatment of breast cancer by network pharmacology
Figure 6. Xiaochaihu decoction induced the apoptosis of CAL-148 and MCF-7 cells. (A, B) Cell death was detected by propidium iodide (PI) assay. (C) The effects of the XCH decoction were evaluated by Hoechst 33342 analysis. (D, E) Cell apoptosis was determined by TUNEL assay. (F) The expression of apoptosis-related proteins was measured by Western blotting. (G) The mRNA levels of Bcl-2, Bcl-xl, and Bax in CAL-148 and MCF-7 cells were evaluated by RT-qPCR. The data are shown as the mean ± SD of three experiments. *P <0.05, **P < 0.01, ***P < 0.001 compared with the control group.