Research Paper Volume 16, Issue 9 pp 7979—7999

Mechanistic exploration and experimental validation of the Xiaochaihu decoction for the treatment of breast cancer by network pharmacology


Figure 7. Xiaochaihu decoction induced the apoptosis of breast cancer cells by inhibiting the PI3K/Akt signaling pathway. (A) The cells were treated with the XCH decoction for 48 hours, and the protein expression of the PI3K/Akt signaling pathway was detected by Western blotting. (B) Cell viability was determined by CCK-8. (C, D) The cells were pretreated with a PI3K agonist (740 Y-P, 25 μg/mL) for 3 hours and then treated with or without the XCH decoction (20 μg/mL) for 48 hours. The expression levels of proteins associated with apoptosis and PI3K/Akt signaling pathway proteins were measured by Western blotting. (E) Knockdown of PI3K in breast cancer cells by lentivirus. (F) CCK-8 was used to detect the effect of the XCH decoction on breast cancer cells after PI3K knockdown. (G, H) Effect of the XCH decoction on apoptosis and PI3K pathway-related protein expression in breast cancer cells after PI3K knockdown. The data are shown as the mean ± SD of three experiments. ***P < 0.001 compared with the control group.