Research Paper Volume 16, Issue 9 pp 8019—8030
AURKB promotes colorectal cancer progression by triggering the phosphorylation of histone H3 at serine 10 to activate CCNE1 expression
Figure 2. AURKB knockdown induced cell cycle arrest in CRC cells. (A, B) The mRNA and protein expression of AURKB was detected in CRC cell lines (HCT116, SW620, SW480, and HT29) using RT-qPCR and western blot. (C, D) HCT116 and SW620 cells were transfected with shNC or shAURKB to detect the mRNA expression of AURKB using RT-qPCR (C) and the protein level of AURKB and pH3S10 using western blot (D). (E–G) AURKB-silenced HCT116 and SW620 cells were subjected to cell proliferation assessment (E), colony formation assay (F), and cell cycle analysis (G). **p < 0.01, ***p < 0.001.