Research Paper Volume 16, Issue 9 pp 8217—8245

Single-cell BCR and transcriptome analysis reveals peripheral immune signatures in patients with thyroid-associated ophthalmopathy


Figure 6. Monocytes displayed increasing inflammatory cytokine production and cos-stimulatory molecule expression in active TAO. (A) tSNE plot showing clusters of monocyte subsets. (B) Pseudotime analysis of monocytes recapitulate known lineage relationships, with CMOs (CD14+S100A8+ S100A9+) branching into either NMOs (FCGR3A+) or IMOs (CD14++ FCGR3A+). (C) FACS plots validated that CMOs, IMOs, and NMOs were present in the peripheral blood. Blood sample from one normal control was used for analysis. (D) Volcano plots showing DEGs of CMOs, IMOs and NMOs in active TAO/NC comparison. (E) Inflammatory cytokines and costimulatory molecules (CD86 and TNF) increased in CMOs and NMOs in active TAO. (F) Representative GO terms enriched by upregulated DEGs of monocytes in active TAO.