Research Paper Volume 17, Issue 7 pp 1834—1851
Sesn-1 is required for lifespan extension during caloric deprivation in C. elegans through inhibition of mTORC1 and activation of autophagy
Figure 2. Role of Sesn-1 in stress resistance. The viability of WT and sesn-1(ok3157) C. elegans was studied under various stress conditions. (A) In axenic M9 medium (n = 783 for WT, n = 437 for sesn-1(ok3157), n = 511 for sesn-1(ie24589), the mean survival rates for sesn-1(ok3157) and sesn-1(ie24589) were 6.13 ± 0.4 and 6.16 ± 0.3 days, respectively, compared to 7.9 ± 0.5 days for WT. The difference between sesn-1(ok3157) and sesn-1(ie24589) was not significant (P > 0.05), while both mutants showed significantly lower survival than WT (P < 0.001). (B) In the presence of 300 μM paraquat (n = 1103 for WT, n = 876 for sesn-1(ok3157), n = 882 for sesn-1(ie24589)), the mean survival rates were 4.1 ± 1.2 and 4.0 ± 0.9 hours for sesn-1(ok3157) and sesn-1(ie24589), respectively, compared to 5.8 ± 1.2 hours for WT. Again, the difference between the two sesn-1 mutants was not significant (P > 0.05), while both were significantly more sensitive than WT (P < 0.001). (C) In the presence of 4 mM H2O2 (n = 1301 for WT, n = 930 for sesn-1(ok3157), n = 827 for sesn-1(ie24589)), the mean survival rates were 3.3 ± 0.5 and 3.8 ± 0.6 hours for sesn-1(ok3157) and sesn-1(ie24589), respectively, compared to 6.4 ± 0.8 hours for WT. The difference between sesn-1(ok3157) and sesn-1(ie24589) was not statistically significant (P > 0.05), while both mutants showed significantly reduced survival compared to WT (P < 0.001). Data are presented as mean ± S.E.M.