Research Paper Volume 9, Issue 6 pp 1585—1594

CircRNA_100269 is downregulated in gastric cancer and suppresses tumor cell growth by targeting miR-630

Yan Zhang 1, *, , Hao Liu 1, *, , Wende Li 2, *, , Jiang Yu 1, , Jin Li 1, , Zhiyong Shen 1, , Gentai Ye 1, , Xiaolong Qi 1, , Guoxin Li 1, ,

  • 1 Department of General Surgery, Nanfang Hospital, Southern Medical University, Guangdong Provincial Engineering Technology Research Center of Minimally Invasive Surgery, Guangzhou, China
  • 2 Guangdong Key Laboratory of Laboratory Animal, Guangdong Laboratory Animal Monitoring Institute, Guangzhou, China
* Co-first authors

received: March 22, 2017 ; accepted: June 20, 2017 ; published: June 27, 2017 ;

https://doi.org/10.18632/aging.101254
How to Cite

Copyright: Zhang et al. This is an open‐access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Although CircRNA_100269 is a biomarker used to predict cancer recurrence, its expression and function in gastric cancer (GC) remain unknown. In this study, the expression of circRNA_100269 and its potential downstream miRNA targets were investigated. The molecular function and regulatory mechanism of circRNA_100269 in GC cell lines were also elucidated. The expression levels of circRNA_100269 and its linear isomer LPHN2 mRNA were found to be downregulated (p<0.01) in GC tissues. The target miRNA was predicted to be miR-630, whose expression was upregulated (p<0.01) and found to be negatively correlated with that of circRNA_100269 (r = -0.688) in GC tissues. Moreover, direct interaction of circRNA_100269 and miR-630 was confirmed through dual-luciferase assays. Overexpressing the circRNA_100269 plasmid inhibited cell proliferation (p<0.05). Furthermore, transfection of miR-630 mimics into cell lines overexpressing circRNA_100269 blocked the function of circRNA_100269 (p<0.05). Thus, circRNA_100269 level was downregulated in GC and correlated negatively with that of miR-630. Taken together, our results suggest that circRNA_100269 and miR-630 comprise a novel pathway that regulates proliferation of GC cells.

Abbreviations

circRNA: circular RNA; miRNA: micro RNA; GC: gastric cancer.