Research Paper Volume 9, Issue 11 pp 2302—2315
Selenium preserves keratinocyte stemness and delays senescence by maintaining epidermal adhesion
- 1 Cell and Tissue Bank of Hospices Civils de Lyon, Hôpital Edouard Herriot, Lyon, France
- 2 Laboratoire de Biologie Tissulaire et Ingénierie Thérapeutique, UMR 5305, CNRS, University Lyon I, Lyon, France
- 3 CarMeN Laboratory, INSERM U-1060, INRA USC-1235, Lyon 1 University, Lyon, France
- 4 Grenoble Alpes University, Grenoble, France
- 5 CEA, INAC, SyMMES, Grenoble, France
- 6 Labcatal Pharmaceuticals, Montrouge, France
received: August 22, 2017 ; accepted: November 2, 2017 ; published: November 25, 2017 ;https://doi.org/10.18632/aging.101322
How to Cite
Copyright: Jobeili et al. This is an open‐access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Skin is constantly exposed to environmental factors such as pollutants, chemicals and ultra violet radiation (UV), which can induce premature skin aging and increase the risk of skin cancer. One strategy to reduce the effect of oxidative stress produced by environmental exposure is the application of antioxidant molecules. Among the endogenous antioxidants, selenoproteins play a key role in antioxidant defense and in maintaining a reduced cellular environment. Selenium, essential for the activity of selenoproteins, is a trace element that is not synthesized by organisms and must be supplied by diet or supplementation. The aim of this study is to evaluate the effect of Selenium supplementation on skin aging, especially on keratinocytes, the main cells of the epidermis. Our results demonstrate for the first time to our knowledge, the major role of Selenium on the replicative life span of keratinocytes and on aging skin. Selenium protects keratinocyte stem cells (KSCs) against senescence via preservation of their stemness phenotype through adhesion to the basement membrane. Additionally, Selenium supplementation maintains the homeostasis of skin during chronological aging in our senescent skin equivalent model. Controlled supplementation with Selenium could be a new strategy to protect skin against aging.
CFE: colony forming efficiency; CFU: colony forming unit; CPD: cumulative population doubling; ECM: extra cellular matrix; EDTA: Ethylenediaminetetraacetic acid; KSCs: keratinocyte stem cells; NaSe: sodium selenite; OCT: optimum cutting temperature; PBS: phosphate buffered saline; PD: population doubling; SE: skin equivalent.