Research Paper Volume 10, Issue 5 pp 1089—1102
Repeated superovulation increases the risk of osteoporosis and cardiovascular diseases by accelerating ovarian aging in mice
- 1 Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, People’s Republic of China
- 2 Maternal and Child Health Hospital of Zigong, Sichuan, People’s Republic of China
- 3 Department of Obstetrics and Gynecology, Hubei Maternity and Child Health Care Hospital, Wuhan, Hubei, People’s Republic of China
- 4 The Central Hospital of Enshi Autonomous Prefecture, Enshi Autonomous Prefecture, Hubei, People’s Republic of China
received: April 2, 2018 ; accepted: May 8, 2018 ; published: May 22, 2018 ; corrected online: September 22, 2018 ;https://doi.org/10.18632/aging.101449
How to Cite
Copyright: Zhang et al. This is an open‐access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Superovulation procedures and assisted reproductive technologies have been widely used to treat couples who have infertility problems. Although generally safe, the superovulation procedures are associated with a series of complications, such as ovarian hyper-stimulation syndrome, thromboembolism, and adnexal torsion. The role of long-term repeated superovulation in ovarian aging and especially in associated disorders such as osteoporosis and cardiovascular diseases is still unclear. In this study, we sought to determine if repeated superovulation by ten cycles of treatment with pregnant mare serum gonadotropin/human chorionic gonadotropin could affect ovarian reserve, ovarian function, bone density and heart function. Ovarian reserve and function were reflected by the size of the primordial follicle pool, anti-Mullerian hormone expressions, hormone levels and fertility status. Furthermore, we examined bone density and heart function by microCT and cardiovascular ultrasonography, respectively. After repeated superovulation, the size of the primordial follicle pool and the expression of anti-mullerian hormone decreased, along with the concentrations of estrogen and progesterone. Mice exposed to repeated superovulation showed an obvious decrease in fertility and fecundity. Furthermore, both bone density and heart ejection fraction significantly decreased. These results suggest that repeated superovulation may increase the risk of osteoporosis and cardiovascular diseases by accelerating ovarian aging.