Research Paper Volume 10, Issue 6 pp 1268—1280
Age-related M1/M2 phenotype changes in circulating monocytes from healthy/unhealthy individuals
- 1 Department of Clinical and Molecular Sciences, DISCLIMO, Università Politecnica delle Marche, Ancona, Italy
- 2 Azienda Ospedaliero Universitaria Ospedali Riuniti, Ancona, Italy
- 3 Clinical and Molecular Laboratory, I.N.R.C.A. (Italian National Research Centre on Aging)-IRCCS, Ancona, Italy
- 4 Department of Experimental, Diagnostic and Specialty Medicine, DIMES, Alma Mater Studiorum, Bologna, Italy
- 5 Biosciences Laboratory, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, Meldola, Italy
- 6 Center of Clinical Pathology and Innovative Therapy, I.N.R.C.A. (Italian National Research Centre on Aging)-IRCCS, Ancona, Italy
- 7 Department of Cardiology, I.N.R.C.A. (Italian National Research Centre on Aging)-IRCCS, Ancona, Italy
received: April 16, 2018 ; accepted: May 30, 2018 ; published: June 8, 2018 ;https://doi.org/10.18632/aging.101465
How to Cite
Copyright: Costantini et al. This is an open‐access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Macrophage polarization is a candidate biomarker of disease-related inflammatory status, but its modulation during aging has not been investigated. To do this, the M1/M2 profile was assessed by CD80/CD163 gating in classical (CD14++CD16-), intermediate (CD14++CD16+), and non-classical (CD14lowCD16+) monocytes from 31 healthy subjects (CTRs) of different ages. Cytofluorimetric analysis showed a significantly different CD80/CD163 distribution in the three subsets, as more than 80% of classical and intermediate monocytes were CD80+CD163+, whereas most non-classical monocytes were CD80-CD163- and CD163+. Non-classical CD163+ monocytes were significantly higher whereas classical CD163+ and CD80-CD163- monocytes significantly lower in older than younger CTRs (cut-off, 65 years), suggesting different age-related trends for M2 subsets. To establish whether an M1/M2 imbalance could be associated with disease, 21 patients with acute myocardial infarction (AMI) were compared with older CTRs. The AMI patients showed a significantly decreased proportion of CD163+CD80+ and an increased proportion of CD163+ and CD163-CD80- cells among classical monocytes, opposite trends to those observed in healthy aging. Moreover, a significantly greater proportion of intermediate and non-classical CD80+ monocytes suggested a shift to a pro-inflammatory phenotype. Overall, CD163/CD80 cytofluorimetric characterization of circulating monocytes provides additional information about their polarization and could be an innovative tool to monitor aging.