Research Paper Volume 11, Issue 15 pp 5351—5367

Long intergenic non-protein coding RNA 511 promotes the progression of osteosarcoma cells through sponging microRNA 618 to upregulate the expression of maelstrom

Wen Guo 1, *, , Qing Yu 1, *, , Ming Zhang 1, , Fang Li 2, , Yu Liu 1, , Weiwei Jiang 1, , Haitao Jiang 1, , Haijun Li 1, ,

  • 1 Department of Orthopaedic Surgery, Taizhou People’s Hospital, Jiangsu 225300, China
  • 2 Department of Neurology, Taizhou Hospital of Traditional Chinese Medicine, Jiangsu 225300, China
* Equal contribution

received: November 22, 2018 ; accepted: July 16, 2019 ; published: August 6, 2019 ;

https://doi.org/10.18632/aging.102109
How to Cite

Copyright © 2019 Guo et al. This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY) 3.0 License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Osteosarcoma is a tumor disease that commonly exists among young populations. Our research explored the role of the LINC00511/microRNA-618/MAEL axis in osteosarcoma.

Expression profiles of long non-coding RNAs (lncRNAs) in osteosarcoma (OS) tissues were constructed, and LINC00511 expression levels were verified with qRT-PCR. LncRNA-miRNA and miRNA-mRNA interactions were predicted. Validation was performed using a dual-luciferase reporter assay. Protein expression levels of MAEL were evaluated by Western blot assays. The effects of LINC00511, miR-618 and MAEL on the proliferation, viability, and metastasis of OS cells were detected using colony formation, cell counting kit-8 (CCK-8) and transwell assays, respectively. The apoptosis rates of OS cells were investigated using flow cytometry. The tumor-suppressing effect of LINC00511 silencing was also analyzed using a xenograft model in nude mice.

LINC00511 overexpression was observed in OS tissues and cell lines. Knockdown of LINC00511 in nude mice inhibited the tumorigenic ability of OS cells. Transfection-induced overexpression of LINC00511 and MAEL, as well as downregulation, highlighted the features of tumor cells, and LINC00511 overexpression reduced apoptosis in vitro.

LINC00511 was confirmed to be beneficial for osteosarcoma development via sponging miR-618 and increasing MAEL expression and may thus be considered a potential target for osteosarcoma therapy.

Abbreviations

ATCC: American Type Culture Collection; CCK-8: cell counting kit-8; FBS: fetal bovine serum; MAEL: maelstrom; miRNAs: microRNAs; OS: osteosarcoma.