Research Paper Volume 11, Issue 18 pp 7880—7898

Mechanisms of ROS-induced mitochondria-dependent apoptosis underlying liquid storage of goat spermatozoa

Tengfei Liu 1, , Yawen Han 1, , Ting Zhou 1, , Ruihang Zhang 1, , Hong Chen 1, , Shulin Chen 1, , Huiying Zhao 1, ,

  • 1 College of Veterinary Medicine, Northwest A & F University, Yangling 712100, Shaanxi Province, China

received: July 20, 2019 ; accepted: September 14, 2019 ; published: September 23, 2019 ;

https://doi.org/10.18632/aging.102295
How to Cite

Copyright © 2019 Liu et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Liquid storage of spermatozoa is important for artificial insemination and herd genetic breeding. However, the extended time of storage inducing the rapid decline in spermatozoa quality limits the development of this technology. The molecular mechanisms underlying liquid storage of spermatozoa remain largely unexplored. In this study, the effects of liquid storage on functional quality of spermatozoa were assessed in goat (Capra hircus). The time-dependent decline in spermatozoa motility showed a strong correlation with the significant increase in apoptosis. Moreover, apoptosis-related ultrastructural changes were observed, especially the defects in mitochondria. A significant decrease in mitochondrial membrane potential and changes in the expression of mitochondrial apoptosis-related proteins indicated mitochondrial dysfunction and mitochondrial apoptotic pathway activation. Notably, the abnormally high level of reactive oxygen species (ROS) caused by liquid storage resulted in oxidative damage to mitochondria and accelerated mitochondria-dependent apoptosis, as demonstrated by the addition of ROS scavenger N-acetylcysteine. Furthermore, critical differentially expressed proteins involved in mitochondria-dependent apoptosis and antioxidant defense were identified and profiled by quantitative proteomic analysis, facilitating the understanding of molecular regulation of ROS-induced mitochondria-dependent apoptosis. These outcomes provide insights into the mechanisms underlying liquid storage of goat spermatozoa and enhance the progress of semen storage technology.

Abbreviations

AI: Artificial insemination; ROS: reactive oxygen species; NAC: N-acetylcysteine; MMP: mitochondrial membrane potential; TMT: tandem mass tag; TEM: transmission electron microscopy; SOD: superoxide dismutase; CAT: catalase; GSH-Px: glutathione peroxidase; MDA: malondialdehyde; DEP: differentially expressed protein; PCA: principal component analysis; PCC: Pearson correlation coefficients; CytC: Cytochrome c.