Research Paper Volume 11, Issue 18 pp 7938—7947

The effects of dietary fatty acids on bone, hematopoietic marrow and marrow adipose tissue in a murine model of senile osteoporosis

Ebrahim Bani Hassan 1, 2, , Mostafa Alderghaffar 3, , Fabien Wauquier 4, , Veronique Coxam 4, , Oddom Demontiero 3, , Sara Vogrin 1, 2, , Yohann Wittrant 4, , Gustavo Duque 1, 2, ,

  • 1 Australian Institute for Musculoskeletal Science (AIMSS), The University of Melbourne and Western Health, St. Albans, VIC, Australia
  • 2 Department of Medicine-Western Health, The University of Melbourne, St. Albans, VIC, Australia
  • 3 Ageing Bone Research Program, Sydney Medical School Nepean, The University of Sydney, Penrith, Australia
  • 4 Human Nutrition Unit, UMR1019, INRA/Université Clermont Auvergne, Clermont-Ferrand, France

received: August 9, 2019 ; accepted: September 14, 2019 ; published: September 25, 2019 ;

https://doi.org/10.18632/aging.102299
How to Cite

Copyright © 2019 Bani Hassan et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Purpose: Marrow adipose tissue (MAT) expansion and associated lipotoxicity are important drivers of age-related bone loss and hematopoietic bone marrow (HBM) atrophy. Fish oil and borage oil (rich in ω3 fatty acids) can partially prevent aged-related bone loss in SAMP8 mice. However, whether preservation of bone mass in this progeria model is associated with MAT volumes remains unknown.

Results: MAT volume fraction (MAT%) showed a negative association with hematopoietic bone marrow (HBM%;r=-0.836, p<0.001) and bone (bone%;r=-0.344, p=0.013) volume fractions.

Adjusting for multiple comparisons, bone% was higher and MAT% was lower in Fish oil (FO)-supplemented groups vs. controls (p<0.001). HBM% did not differ significantly between the four groups. However, in the group supplemented with FO, HBM comprised higher fractions and MAT constituted lower fractions of total marrow vs. controls (p<0.001).

Conclusion: Feeding FO-enriched diet prevented age-related bone and HBM loss, by reducing MAT expansion. Our results further emphasize on the role(s) of MAT expansion in bone and HBM atrophy.

Methods: SAMP8 mice (n>9 /group) were allocated into 4 categories and fed a control ration, FO-, sunflower oil (SFO)- and borage oil-enriched diets for lifetime. Femurs were scanned using microcomputed tomography (μCT) and bone, MAT, and HBM volumes were determined using an image analysis software.

Abbreviations

MAT: Marrow adipose tissue; HBM: hematopoietic bone marrow; FO: fish oil; SFO: sunflower oil; μCT: microcomputed tomography; IL: interleukin.