Research Paper Volume 12, Issue 2 pp 1186—1200

Fibroblast growth factor 10 alleviates particulate matter-induced lung injury by inhibiting the HMGB1-TLR4 pathway

Lingjing Liu1, *, , Chenjian Song2, *, , Jingli Li1, , Qiang Wang1, , Mingyang Zhu1, , Yiran Hu1, , Junjie Chen1, , Chaolei Chen1, , Jin-San Zhang3, , Nian Dong1, , Chengshui Chen1, ,

  • 1 Department of Pulmonary Medicine, Wenzhou Medical University First Affiliated Hospital, Wenzhou 325006, China
  • 2 Department of Pulmonary Medicine, Yiwu Central Hospital, Yiwu 322000, China
  • 3 Department of Pharmacy, Wenzhou Medical University Pharmacy School, Wenzhou 325006, China
* Equal contribution

Received: October 2, 2019       Accepted: December 25, 2019       Published: January 20, 2020
How to Cite

Copyright: © 2020 Liu et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Exposure to particulate matter (PM) is associated with increased incidence of respiratory diseases. The present study aimed to investigate the roles of fibroblast growth factor 10 (FGF10) in PM-induced lung injury. Mice were intratracheally instilled with FGF10 or phosphate-buffered saline at one hour before instillation of PM for two consecutive days. In addition, the anti-inflammatory impact of FGF10 in vitro and its effect on the high-mobility group box 1 (HMGB1)-toll-like receptor 4 (TLR4) pathway was investigated. It was found that PM exposure is associated with increased inflammatory cell infiltration into the lung and increased vascular protein leakage, while FGF10 pretreatment attenuated both of these effects. FGF10 also decreased the PM-induced expression of interleukin (IL)-6, IL-8, tumor necrosis factor-α and HMGB1 in murine bronchoalveolar lavage fluid and in the supernatants of human bronchial epithelial cells exposed to PM. FGF10 exerted anti-inflammatory and cytoprotective effects by inhibiting the HMGB1-TLR4 pathway. These results indicate that FGF10 may have therapeutic values for PM-induced lung injury.


PM: Particulate matter; FGF10: Fibroblast Growth Factor 10; IL-6: Interleukin-6; IL-8: Interleukin-8; TNF-α: Tumor necrosis factor-α; BALF: Bronchoalveolar lavage fluid; HMGB1: High mobility group box 1 protein; TLR4: Toll-like receptor 4; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; PBS: Phosphate buffered saline; RPMI-1640: Roswell Park Memorial Institute 1640; qRT-PCR: Quantitative reverse transcriptase-polymerase chain reaction.