COVID-19 Research Paper Volume 12, Issue 8 pp 6536—6542

Anti-SARS-CoV-2 virus antibody levels in convalescent plasma of six donors who have recovered from COVID-19

Libo Zhang 1, *, , Rongrong Pang 1, *, , Xiang Xue 2, , Jingjing Bao 1, , Sheng Ye 3, , Yudong Dai 4, , Yishan Zheng 5, , Qiang Fu 4, , Zhiliang Hu 6, 7, , Yongxiang Yi 6, ,

  • 1 Department of Laboratory Medicine, Nanjing Red Cross Blood Center, Nanjing 210003, Jiangsu, China
  • 2 Department of Biochemistry and Molecular Biology, University of New Mexico, Albuquerque, NM 87131, USA
  • 3 Department of Apheresis, Nanjing Red Cross Blood Center, Nanjing 210003, Jiangsu, China
  • 4 Department of Blood Management, Administrative Office, Nanjing Red Cross Blood Center, Nanjing 210003, Jiangsu, China
  • 5 Department of Critical Medicine, The Second Hospital of Nanjing, Nanjing University of Chinese Medicine, Nanjing 210003, Jiangsu, China
  • 6 Nanjing Infectious Disease Center, The Second Hospital of Nanjing, Nanjing University of Chinese Medicine, Nanjing 210003, Jiangsu, China
  • 7 School of Public Health, Nanjing Medical University, Nanjing 211166, Jiangsu, China
* Co-first authors

received: March 27, 2020 ; accepted: April 14, 2020 ; published: April 22, 2020 ;

https://doi.org/10.18632/aging.103102
How to Cite

Copyright © 2020 Zhang et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Background: Anti-SARS-CoV-2 virus antibody levels in convalescent plasma (CP), which may be useful in severe Anti-SARS-CoV-2 virus infections, have been rarely reported.

Results: A total of eight donors were considered for enrollment; two of them were excluded because of ineligible routine check. Of the six remaining participants, five samples were tested weakly positive by the IgM ELISA. Meanwhile, high titers of IgG were observed in five samples. The patient treated with CP did not require mechanical ventilation 11 days after plasma transfusion, and was then transferred to a general ward.

Conclusions: Our serological findings in convalescent plasma from recovered patients may help facilitate understanding of the SARS-CoV-2 infection and establish CP donor screening protocol in COVID-19 outbreak.

Methods: Anti-SARS-CoV-2 antibodies including IgM and IgG were measured by two enzyme-linked immunosorbent assays (ELISA) in convalescent plasma from six donors who have recovered from coronavirus disease 2019 (COVID-19) in Nanjing, China. CP was also utilized for the treatment of one severe COVID-19 patient.

Abbreviations

CP: convalescent plasma; COVID-19: coronavirus disease 2019; WBC: white blood cell counts; ALT: alanine aminotransferase; CK: creatine kinase; LDH: lactate dehydrogenase; PT: prothrombin time; IL-6: interleukin 6; CRP: C-reactive protein; NA: not available; OD: optical density; ICU: Intensive Care Unit.