Research Paper Advance Articles

Human amnion-derived mesenchymal stem cells promote osteogenic differentiation of human bone marrow mesenchymal stem cells via H19/miR-675/APC axis

Xiaojie Ma1,2, *, , Yifeng Bian1,2, *, , Hua Yuan1,2, , Ning Chen1,2, , Yongchu Pan1,3, , Weina Zhou1,4, , Shiyu Gao1,2, , Xin Du5, , Shushu Hao1, , Zixin Yan1, , Xuan Li1, , Keyue Liu1, , Fan Xu1, , Yuli Wang1,2, , Yifei Du1,2, ,

  • 1 Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, China
  • 2 Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing, China
  • 3 Department of Orthodontics, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing, China
  • 4 Department of Temporomandibular Joint, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing, China
  • 5 State Key Laboratory of Bioelectronics, Southeast University, Nanjing, China
* Equal contribution

Received: December 16, 2019       Accepted: April 17, 2020       Published: May 20, 2020      

https://doi.org/10.18632/aging.103277
How to Cite

Copyright © 2020 Ma et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Bone volume inadequacy is an emerging clinical problem impairing the feasibility and longevity of dental implants. Human bone marrow mesenchymal stem cells (HBMSCs) have been widely used in bone remodeling and regeneration. This study examined the effect of long noncoding RNAs (lncRNAs)-H19 on the human amnion-derived mesenchymal stem cells (HAMSCs)-droved osteogenesis in HBMSCs. HAMSCs and HBMSCs were isolated from abandoned amniotic membrane samples and bone marrow. The coculture system was conducted using transwells, and H19 level was measured by quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR). The mechanism was further verified. We here discovered that osteogenesis of HBMSCs was induced by HAMSCs, while H19 level in HAMSCs was increased during coculturing. H19 had no significant effect on the proliferative behaviors of HBMSCs, while its overexpression of H19 in HAMSCs led to the upregulated osteogenesis of HBMSCs in vivo and in vitro; whereas its knockdown reversed these effects. Mechanistically, H19 promoted miR-675 expression and contributed to the competitively bounding of miR-675 and Adenomatous polyposis coli (APC), thus significantly activating the Wnt/β-catenin pathway. The results suggested that HAMSCs promote osteogenic differentiation of HBMSCs via H19/miR-675/APC pathway, and supply a potential target for the therapeutic treatment of bone-destructive diseases.

Abbreviations

lncRNA: long noncoding RNA; HAMSCs: human amnion-derived mesenchymal stem cells; HBMSCs: human bone marrow mesenchymal stem cells; RT-PCR: quantitative real-time reverse transcription-polymerase chain reaction; APC: Adenomatous polyposis coli; ALP: alkaline phosphatase; Micro-CT: micro-computerized tomography; MSCs: mesenchymal stem cells; HASCs: human adipose-derived stem cells; MiRNAs: microRNAs; SSRO: sagittal split ramus osteotomy; DMEM: dulbecco’s modified eagle medium; FBS: fetal bovine serum; GFP: green fluorescent reporter gene; POL: polybrene; OCN: osteocalcin; OSX: osterix; BSA: bovine serum albumin; H&E: hematoxylin and eosin; SD: standard deviation; ANOVA: analysis of variance.