Research Paper|Volume 12, Issue 17|pp 17114—17121

miR-345-5p regulates adipogenesis via targeting VEGF-B

Xiaofeng Liu¹, Yang He², Zhaolan Feng¹, Jianjian Sheng¹, Aiwu Dong¹, Meiying Zhang³, Lingling Cao¹

¹Department of Endocrinology, The First Hospital of Jiujiang City, Jiujiang 332000, China
²Department of Endocrinology, Zhuhai People’s Hospital (Zhuhai Hospital affiliated with Jinan University), Zhuhai 519000, China
³The Second Affiliated Hospital of Nanchang University, Nanchang 330000, China

* Equal contribution

Received: March 17, 2020Accepted: June 19, 2020Published: September 14, 2020

Copyright: © 2020 Liu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Adipocyte differentiation involves a series of highly synergistic processes, including clone amplification, proliferation arrest, and terminal differentiation. However, the mechanisms that control these different steps remain unclear. Emerging studies support that miRNAs play an important role in regulating adipogenesis. In this study, we found that the expression of miR-345-5p decreased during adipogenic differentiation, and overexpression of miR-345-5p reduced lipid accumulation in adipocytes and the expression of adipocyte related genes essential to lipogenic transcription, fatty acid synthesis and fatty acid transport. In addition, miR-345-5p directly targeted the 3’UTR of vascular endothelial growth factor B, and miR-345-5p mimic inhibited the expression of vascular endothelial growth factor B at both mRNA and protein levels. In conclusion, our results demonstrate that miR-345-5p inhibits adipocyte differentiation via targeting vascular endothelial growth factor B.