Research Paper Volume 13, Issue 1 pp 89—103

Further assessment of Salvia haenkei as an innovative strategy to counteract skin photo-aging and restore the barrier integrity

Veronica Cocetta1, , Jessica Cadau1, , Miriam Saponaro2,3, , Isabella Giacomini1, , Stefano Dall’Acqua1, , Stefania Sut1, , Daniela Catanzaro1, , Genny Orso1, , Giorgia Miolo1, , Luca Menilli1, , Andrea Pagetta1, , Eugenio Ragazzi1, , Monica Montopoli1,2, ,

  • 1 Department of Pharmaceutical and Pharmacological Sciences, University of Padova, Padova 35121, Italy
  • 2 Veneto Institute of Molecular Medicine (VIMM), Padova 35121, Italy
  • 3 Department of Medicine, University of Padova, Padova 35128, Italy

Received: December 13, 2019       Accepted: December 9, 2020       Published: January 8, 2021
How to Cite

Copyright: © 2021 Cocetta et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Skin is the essential barrier of the human body which performs multiple functions. Endogenous factors, in concert with external assaults, continuously affect skin integrity, leading to distinct structural changes that influence not only the skin appearance but also its various physiological functions. Alterations of the barrier functions lead to an increased risk of developing disease and side reactions, thus the importance of maintaining the integrity of the epidermal barrier and slowing down the skin aging process is evident.

Salvia haenkei (SH) has been recently identified as a potential anti-senescence agent; its extract is able to decrease the level of senescent cells by affecting the IL1α release and reducing reactive oxygen species (ROS) generation.

In this study, SH extract was tested on human keratinocyte cell line (HaCaT) exposed to stress factors related to premature aging of cells such as free radicals and ultraviolet B radiation. We confirmed that SH acts as scavenger of ROS and found its ability to restore the skin barrier integrity by reinforcing the cytoskeleton structure, sealing the tight junctions and increasing the migration rate of cells.

Given these results, this work becomes relevant, identifying Salvia haenkei as a compound useful for anti-aging skin treatment in clinical performance.


AJ: adherens junction; ECM: extracellular matrix; FLG: filaggrin; H2-DCF-DA: 2′,7′-dichlorofluorescin-diacetate; H2O2: Hydrogen Peroxide; MMP: matrix metalloproteinase; NAC: N-acetylcysteine; NMF: natural moisturizing factor; ROS: reactive oxygen species; SIRT1: Sirtuin1; SH: Salvia haenkei; TJ: tight junction; UVB: Ultraviolet radiation B.