Research Paper Volume 13, Issue 1 pp 89—103
Further assessment of Salvia haenkei as an innovative strategy to counteract skin photo-aging and restore the barrier integrity
- 1 Department of Pharmaceutical and Pharmacological Sciences, University of Padova, Padova 35121, Italy
- 2 Veneto Institute of Molecular Medicine (VIMM), Padova 35121, Italy
- 3 Department of Medicine, University of Padova, Padova 35128, Italy
Received: December 13, 2019 Accepted: December 9, 2020 Published: January 8, 2021
https://doi.org/10.18632/aging.202464How to Cite
Copyright: © 2021 Cocetta et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Skin is the essential barrier of the human body which performs multiple functions. Endogenous factors, in concert with external assaults, continuously affect skin integrity, leading to distinct structural changes that influence not only the skin appearance but also its various physiological functions. Alterations of the barrier functions lead to an increased risk of developing disease and side reactions, thus the importance of maintaining the integrity of the epidermal barrier and slowing down the skin aging process is evident.
Salvia haenkei (SH) has been recently identified as a potential anti-senescence agent; its extract is able to decrease the level of senescent cells by affecting the IL1α release and reducing reactive oxygen species (ROS) generation.
In this study, SH extract was tested on human keratinocyte cell line (HaCaT) exposed to stress factors related to premature aging of cells such as free radicals and ultraviolet B radiation. We confirmed that SH acts as scavenger of ROS and found its ability to restore the skin barrier integrity by reinforcing the cytoskeleton structure, sealing the tight junctions and increasing the migration rate of cells.
Given these results, this work becomes relevant, identifying Salvia haenkei as a compound useful for anti-aging skin treatment in clinical performance.
Abbreviations
AJ: adherens junction; ECM: extracellular matrix; FLG: filaggrin; H2-DCF-DA: 2′,7′-dichlorofluorescin-diacetate; H2O2: Hydrogen Peroxide; MMP: matrix metalloproteinase; NAC: N-acetylcysteine; NMF: natural moisturizing factor; ROS: reactive oxygen species; SIRT1: Sirtuin1; SH: Salvia haenkei; TJ: tight junction; UVB: Ultraviolet radiation B.