Research Paper Volume 13, Issue 5 pp 7314—7329
Levodopa affects spike and local field synchronisation in the pedunculopontine nucleus of a rat model of Parkinson’s disease
- 1 Experimental Centre, Shandong University of Traditional Chinese Medicine, Ji’nan 250355, Shandong, China
- 2 Key Laboratory of Vector Biology and Pathogen Control of Zhejiang, School of Medicine, Huzhou University, Huzhou Central Hospital, Huzhou 313000, Zhejiang, China
- 3 Department of Traditional Chinese Medicine, Shandong University of Traditional Chinese Medicine, Ji’nan 250355, Shandong, China
Received: November 4, 2020 Accepted: January 4, 2021 Published: February 26, 2021
https://doi.org/10.18632/aging.202585How to Cite
Copyright: © 2021 Zhang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
The pedunculopontine nucleus (PPN) undergoes significant anatomic and electrophysiological alterations in Parkinson’s disease (PD), severely impacting locomotion. However, the effect of 6-hydroxydopamine (6-OHDA) lesion and levodopa (L-DOPA) therapy on the relationships between spike activities and local field potential (LFP) within the PPN is not well-understood. Synchronisation between the spike activity of individual neurones and LFP of neuronal ensembles is a crucial problem in the pathogenesis of PD. In this study, LFP signals and spikes in the PPN of rats in control, lesioned, and L-DOPA groups were recorded synchronously with a multi-unit electrical signal acquisition system and analysed for their coherence value, spike-field coherence, and phase-lock relationship. The spike-LFP relationship in the PPN was markedly increased in specific frequency bands because of the 6-OHDA lesion but differed depending on the animal locomotion state and neuronal type. L-DOPA had a limited therapeutic effect on the 6-OHDA-induced increase in the coherence value. Our study demonstrates that the PPN spike-LFP relationship is involved in the pathogenesis of PD and is critical for the effects of L-DOPA, providing a basis for the clinical treatment of refractory PD symptoms.
Abbreviations
APO: apomorphine; DBS: deep brain stimulation; ISI: inter-spike intervals; L-DOPA: levodopa; LFP: local field potential; 6-OHDA: 6-hydroxydopamine; PCA: principal component analysis; PD: Parkinson’s disease; PPN: pedunculopontine nucleus; SFC: spike-field coherence; STP: spike-triggered power; TH: tyrosine hydroxylase.