Research Paper Volume 13, Issue 7 pp 9874—9899
DNASE1L3 arrests tumor angiogenesis by impairing the senescence-associated secretory phenotype in response to stress
- 1 Department of Hepatobiliary Surgery, Zhongnan Hospital of Wuhan University, Wuhan 430071, P.R. China
- 2 Department of Hepatobiliary Surgery, Tianjin Medical University General Hospital, Tianjin 300052, P.R. China
Received: December 18, 2020 Accepted: February 19, 2021 Published: March 19, 2021https://doi.org/10.18632/aging.202740
How to Cite
Copyright: © 2021 Guo et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Hepatocellular carcinoma (HCC) is one of the most challenging and aggressive cancers with limited treatment options because of tumor heterogeneity. Tumor angiogenesis is a hallmark of HCC and is necessary for tumor growth and progression. DNA damage stress and its associated deoxyribonuclease1-like 3 (DNASE1L3) are involved in HCC progression. Here, we explored the influence mechanism of DNASE1L3 on tumor angiogenesis under DNA damage stress in vitro and in vivo. DNASE1L3 was found downregulated and negatively correlated with poor prognosis of resectable and unresectable HCC patients. The tissue microarray of HCC revealed the negative association between DNASE1L3 and cancer vasculature invasion. Mechanistically, DNASE1L3 was found to relieve cytoplasmic DNA accumulation under DNA damage stress in HCC cell lines, in turn cell senescence and senescence-associated secretory phenotype were arrested via the p53 and NF-κB signal pathway, and hence, tumor angiogenesis was impaired. Furthermore, we found that DNASE1L3 excised these functions by translocating to the nucleus and interacting with H2BE under DNA damage stress using co-immunoprecipitation and fluorescence resonance energy transfer assay. In conclusion, DNASE1L3 inhibits tumor angiogenesis via impairing the senescence-associated secretory phenotype in response to DNA damage stress.
HCC: Hepatocellular carcinoma; DNASE1L3: deoxyribonuclease1-like 3; HBV: hepatitis B virus; HCV: hepatitis C virus; DDR: DNA damage response; NLS: nuclear localization signal; AWERB: Animal Welfare and Ethical Review Body; IHC: immunohistochemistry; UV: ultraviolet; EdU: 5-Ethynyl-2′-deoxyuridine; FRET: Fluorescence resonance energy transfer; TCGA: The Cancer Genome Atlas; qPCR: quantitative real-time PCR; DDF: DNA damage foci; RCD: regulation of cell death.