Research Paper Volume 13, Issue 6 pp 9028—9042

Effect of bovine bone collagen oligopeptides on wound healing in mice

Di Li1, , Jin-wei Ren2, , Teng Xu2, , Lin Li2, , Peng Liu1, , Yong Li2, ,

  • 1 Department of Clinical Nutrition, Peking University People’s Hospital, Beijing 100044, China
  • 2 Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100191, China

Received: November 28, 2020       Accepted: February 8, 2021       Published: March 10, 2021
How to Cite

Copyright: © 2021 Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Impaired wound healing often brings a set of problems in clinical practice. This study aimed to observe the wound healing potential of bovine bone collagen oligopeptides (BCOP) in mice. After an operation, mice in BCOP-treated groups were given intragastric administration of BCOP, while others were administered vehicle. Mice were sacrificed at different points. The wound healing condition and the tensile strength were observed, serum biochemical indexes and mRNA expression of level of related genes were measured. Compared with the normal control group, albumin (ALB), prealbumin (PA), transferrin (TRF), hydroxyproline (Hyp) levels and tension strength in the BCOP-treated groups increased significantly (p < 0.05). A pathological report showed that neutrophil granulocyte in the BCOP-treated groups decreased, while blood capillary and fibroblasts increased. The levels of serum inflammation indexes like interleukin (IL)-8, tumor necrosis factor (TNF)-α, chemokine (C-C motif) ligand 2 (CCL2) and C-reactive protein (CRP) significantly decreased in full-thickness incision model, whereas increased in full-thickness excision model (p < 0.05). Furthermore, IL-10, stromal cell-derived factor-1 alpha (SDF-1α) levels and the mRNA expression of vascular endothelial growth factor (VEGF) significantly increased in both models (p < 0.05). These results suggested that oral administration of BCOP could promote wound healing in mice.


ALB: Albumin; BCOP: Bovine bone collagen oligopeptides; HE: Hematoxylin-eosin staining; CRP: High-sensitivity C-reactive protein; IL-8: Interleukin-8; IL-10: Interleukin-10; CCL2: chemokine (C-C motif) ligand 2; PA: Prealbumin; SDF-1α: stromal cell-derived factor-1 alpha; TNF-α: Tumor necrosis factor-alpha; TRF: Transferrin; VEGF: Vascular endothelial growth factor; PI3K: Phosphatidylinositol 3-kinase; mTOR: Mammalian target of rapamycin.