Research Paper Volume 13, Issue 8 pp 11629—11645
Circular RNA hsa_circ_0072309 promotes tumorigenesis and invasion by regulating the miR-607/FTO axis in non-small cell lung carcinoma
- 1 Department of General Surgery, Changzhou Seventh People's Hospital, Changzhou 213011, Jiangsu, China
- 2 Department of General Surgery, Changzhou Geriatric Hospital Affiliated to Soochow University, Changzhou 213011, Jiangsu, China
- 3 Department of Oncology, Changzhou Seventh People's Hospital, Changzhou 213011, Jiangsu, China
- 4 Department of Traumatology, Changzhou Seventh People's Hospital, Changzhou 213011, Jiangsu, China
Received: November 2, 2020 Accepted: March 4, 2021 Published: April 20, 2021
https://doi.org/10.18632/aging.202856How to Cite
Copyright: © 2021 Mo et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Emerging evidence has demonstrated that circular RNAs (circRNAs) are abnormally expressed in non-small cell lung carcinoma (NSCLC). However, the contributions of circRNAs to the tumorigenesis of lung adenocarcinoma (LUAD), one of the subtypes of NSCLC, remain unclear. Based on a microarray assay, we found that hsa_circ_0072309 was significantly upregulated in NSCLC compared with matched normal samples. Moreover, functional experiments demonstrated that hsa_circ_0072309 promotes the proliferation, migration, and invasion of NSCLC cells. In vitro precipitation of circRNAs, luciferase reporter assays, and biotin-coupled microRNA capture assays were carried out to investigate the mechanisms by which hsa_circ_0072309 regulates NSCLC. Through the above work, we found that hsa_circ_0072309 interacted with miR-607 via its miRNA response element to upregulate the expression of FTO, an m6A demethylase and downstream target of miR-607, thus promoting tumorigenesis of NSCLC. In total, our findings indicated the oncogenic role of hsa_circ_0072309 in NSCLC and provide a potential target for treatment.