Research Paper Volume 13, Issue 9 pp 12378—12394
Transcriptomic profile of the mice aging lung is associated with inflammation and apoptosis as important pathways
- 1 Division of Pulmonary Allergy and Critical Care Medicine, Aging Institute, Department of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15219, USA
- 2 Division of Pulmonary, Critical Care and Sleep Medicine, Department of Internal Medicine, Davis Heart and Lung Research Institute, Ohio State University, Columbus, Ohio 43210, USA
- 3 Instituto Nacional de Enfermedades Respiratorias “Ismael Cosío Villegas”, Ciudad de México 14080, México
- 4 Facultad de Ciencias, Universidad Nacional Autónoma de México, Ciudad de México 04510, México
Received: September 21, 2020 Accepted: April 29, 2021 Published: May 12, 2021https://doi.org/10.18632/aging.203039
How to Cite
Copyright: © 2021 Calyeca et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Aging is a universal biological process characterized by a progressive deterioration in functional capacity and an increased risk of morbidity and mortality over time. In the lungs, there are considerable changes in lung structure and function with advancing age; however, research on the transcriptomic profile implicated in this process is scanty. In this study, we addressed the lung transcriptome changes during aging, through a global gene expression analysis of normal lungs of mice aged 4- and 18-months old. Functional pathway enrichment analysis by Ingenuity Pathway Analysis (IPA) revealed that the most enriched signaling pathways in aged mice lungs are involved in the regulation of cell apoptosis, senescence, development, oxidative stress, and inflammation. We also found 25 miRNAs significantly different in the lungs of old mice compared with their younger littermates, eight of them upregulated and 17 downregulated. Using the miRNet database we identified TNFα, mTOR, TGFβ, WNT, FoxO, Apoptosis, Cell cycle, and p53 signaling pathways as the potential targets of several of the dysregulated miRNAs supporting that old lungs have increased susceptibility for apoptosis, inflammation, and fibrosis. These findings reveal differential expression profiles of genes and miRNAs affecting cell survival and the inflammatory response during lung aging.