Research Paper Advance Articles

CXCR5 induces perineural invasion of salivary adenoid cystic carcinoma by inhibiting microRNA-187

Mei Zhang1, , Jia-Shun Wu1, , Hong-Chun Xian1, , Bing-Jun Chen1, , Hao-Fan Wang1, , Xiang-Hua Yu1, , Xin Pang1, , Li Dai1, , Jian Jiang2, , Xin-Hua Liang1, , Ya-Ling Tang1, ,

  • 1 State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology (Sichuan University), Chengdu 610041, China
  • 2 Department of Head and Neck Surgery, Sichuan Cancer Hospital, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, Sichuan, China

Received: December 3, 2020       Accepted: March 11, 2021       Published: June 10, 2021      

https://doi.org/10.18632/aging.203097
How to Cite

Copyright: © 2021 Zhang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

CXCR5 played critical roles in tumorigenesis and metastasis. Nevertheless, little was known about the involvement of CXCR5 in perineural invasion (PNI) of salivary adenoid cystic carcinoma (SACC). Here, we confirmed upregulation of CXCR5 in SACC specimens and cells and identified that CXCR5 exhibited a significant positive correlation with PNI. Functionally, knockdown of CXCR5 suppressed SACC cells migration, invasion and PNI ability, whereas CXCR5 overexpression displayed the opposite effects. Moreover, CXCR5 downregulated microRNA (miR)-187, which could competitively sponge S100A4. The PNI-inhibitory effect of CXCR5 knockdown or miR-187 overexpression could be reversed by elevated expression of S100A4. Conjointly, our data revealed that CXCR5 facilitated PNI through downregulating miR-187 to disinhibit S100A4 expression in SACC.

Abbreviations

PNI: Perineural invasion; SACC: Salivary adenoid cystic carcinoma; miR-187: MicroRNA-187; GPCR: G-protein coupled receptors; RISC: RNA-induced silencing complex; S100A4: S100 calcium binding protein A4; p75NTR: P75 neurotrophin receptor; GFAP: Glial fibrillary acidic protein; IHC: Immunohistochemistry; DMEM: Dulbecco’s Modified Eagle’s Medium; FBS: Fetal bovine serum; DAPI: Diamidino-2-phenylindole; qRT-PCR: Quantitative real-time PCR.