Research Paper Volume 13, Issue 24 pp 26095—26117

Knockdown hsa_circ_0063526 inhibits endometriosis progression via regulating the miR-141-5p / EMT axis and downregulating estrogen receptors

Zhangming Wei1,2,3, *, , Yi Hu4, *, , Xiang He1,2,3, *, , Mengmeng Zhang3, , Xinyue Zhang3, , Yali Wang5, , Xiaoling Fang3, , Liping Li1,2, ,

  • 1 Department of Obstetrics, The Second Clinical Medical College, Jinan University, Shenzhen People's Hospital, Shenzhen 518020, Guangdong, China
  • 2 The First Affiliated Hospital, Jinan University, Guangzhou 510000, Guangdong, China
  • 3 Department of Obstetrics and Gynecology, The Second Xiangya Hospital, Central South University, Changsha 410000, Hunan, P.R. China
  • 4 The First Affiliated Hospital, Department of Obstetrics and Gynaecology, Hengyang Medical School, University of South China, Hengyang 421001, Hunan, China
  • 5 Xiangya Stomatological Hospital, Central South University, Changsha 410008, Hunan, China
* Equal contribution

Received: March 22, 2021       Accepted: December 8, 2021       Published: December 30, 2021
How to Cite

Copyright: © 2021 Wei et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Endometriosis can cause severe social burdens. Abnormal circular RNA levels have been found to lead to changes of related gene expression, thereby mediating the occurrence and development of a series of diseases, including endometriosis. The role of circRNA in endometriosis is still in its infancy. This study will explore the role of circRNA hsa_circ_0063526 with microRNA-141-5p in the development of endometriosis. The expression levels of genes were detected by RT-qPCR. Transwell, wound-healing, and EdU assays were performed on the End1 / E6E7 cell line from the endometriosis patient. PCR and immunohistochemistry were used to detect the expression of candidate regulatory genes in ectopic lesions in an endometriosis mice model. The expression level of hsa_circ_0063526 in ectopic tissue of endometriosis patients was significantly higher than control (P<0.05), The expression levels of hsa_circ_0063526 and miRNA-141-5P in ectopic tissue of endometriosis were negatively correlated (P<0.05). Knockdown of hsa_circ_0063526 inhibited the invasion, migration, and proliferation ability of End1 / E6E7 cell; the inhibition of microRNA-141-5p rescued this inhibition (P <0.05). In vivo experiments showed that miR-141-5p and si-hsa_circ_0063526 treatment reduced lesion size and regulated endometriosis genes. Our data suggest that hsa_circ_0063526 and miR-141-5p are possible biomarkers and therapeutic targets for endometriosis.


EMs: endometriosis; N-cad: N-cadherin; E-cad: E-cadherin; EMT: Epithelial-mesenchymal transition; ncRNAs: non-coding RNA; circRNA: circular RNA; miRNA: microRNA; RNA: Ribonucleic Acid; ZO-1: Zona occludens 1; PVDF: polyvinylidene difluoride; 3'UTR: 3'- untranslated region; ceRNA: competing endogenous RNA; BS: back splicing; RNAi: RNA interference; siRNA: small interference RNA; EdU: 5-acetylene 2' -deoxyuridine; PBS: phosphate buffer saline; VEGF: Vascular endothelial growth factor.