Research Paper Volume 15, Issue 6 pp 1878—1889
A multi-omics approach based on 1H-NMR metabonomics combined with target protein analysis to reveal the mechanism of RIAISs on cervical carcinoma patients
- 1 Tianjin Medcical University Cancer Institute and Cancer Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin's Clinical Research Center for Cancer, Tianjin 300060, P.R. China
- 2 Department of Radiotherapy Oncology, The First Affiliated Hospital of Xi'an Jiao Tong University, Xi'an 710061, Shaanxi, P.R. China
- 3 The Gao Qing People's Hospital of Zibo, Zibo 255000, Shandong, P.R. China
Received: June 9, 2022 Accepted: September 1, 2022 Published: September 27, 2022
https://doi.org/10.18632/aging.204305How to Cite
Copyright: © 2022 Yanlan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Cervical carcinoma (CC) is the fourth most common cancer in females and radiotherapy is always as the definitive therapy for cervical cancer patients who are not suitable for surgery. Radiation-induced acute intestinal symptoms (RIAISs) occur in 50-80% of cervical cancer patients. Some research shows that RIAISs may relate to inflammatory reaction by radiotherapy but the action mechanism is also not clearly and the details of the molecular mechanism are still urgently needed. In this paper, basing on 1H-NMR metabonomic and bioinformatics analysis, an integrated multi-omics analysis including metabonomics and bioinformatics was performed. We propose a hypothesis about pathogenic mechanism on RIAISs and proofed it through western-blot. Our results indicated significant dysregulation of metabolic pathways in RIAIS patients. Most importantly, we found that RIAISs were associated p53 and PI3K-AKT pathway.
Abbreviations
CC: Cervical carcinoma; RIAISs: Radiation-induced acute intestinal symptoms; RIID: Radiationinduced intestinal damage; NMR: Nuclear magnetic resonance; FDR: False discovery rate.